Noticias y artículos de Zeulab

Protein phospatase (PP2A) from ZEU-INMUNOTEC is the best choice for okadaic acid and microcystins detection

14/10/11 13:21 / Escrito por ZEULAB S.L.

Protein phospatase (PP2A) from ZEU-INMUNOTEC is the best choice for okadaic acid and microcystins detection

Recently, two papers have been published in Talanta and Analitical Chimica Acta comparing protein phosphatases (PP) from several sources (ZEU-INMUNOTEC, Millipore and GTP) and origin (recombinant or natural).

 A colorimetric in vitro assay was used to compare phosphatases in solution or entrapped within either a photoploymer or an agarose. Authors showed that the phosphatase from ZEU was the most sensitive to microcystins with a detection limit of 0.0039 ug/L and an IC50 of 0.56 ug/L for MC-LR in the assay conditions.

 In the case of okadaic acid toxins, phosphatase from ZEU had a detection limit of 0.012 ug/L when phosphatase was used in solution. Analysis of real samples (mussels) using the ZEU´s phosphatase showed also a very good repeatability (RSD = 1-6%).

 These results show that phosphatase from ZEU is the best choice for the detection of okadaic acid and microcystin toxins.

 ZEU-INMUNOTEC uses its own phosphatase to produce OKATEST (a kit for the detection of okadaic acid toxins in molluscs, recently UE-validated) and MICROCYSTEST (a kit for the detection of microcystins in water, evaluated under the ETV program in agreement with the U.S. Environmental Protection Agency).

ZEULAB S.L.

Escrito por ZEULAB S.L.