Single Laboratory Validation of A Ready-to-Use Phosphatase Inhibition Assay for Detection of Okadaic Acid Toxins
Toxins.
2012, 4, 339-352;
http://www.mdpi.com/2072-6651/4/5/339/pdf
A SLV of a PP2A developed by ZEU-INMUNOTEC, just published on Toxins journal
May 3, 2012 9:33:45 AM / by admin posted in DSP toxins, Marine biotoxins, Marine toxins, OA toxins, okadaic acid, Okatest, phosphatase, PP2A, protein phosphatases, SLV
A comparison of assay techniques for Okadaic acid toxins determination in shellfish
Jan 23, 2012 9:40:16 AM / by admin posted in DSP toxins, Marine biotoxins, Marine toxins, okadaic acid, Okatest
PP2A (protein phosphatase) technique appears satisfactory for the analysis of DSP toxins in shellfish and is recommended as a rapid assay method for screening and end product testing.
This is one of the conclusions raised from a study carried out within the water project http://www.nppwater.com/index.php
Mussel samples from the biotoxins monitoring program carried by the Irish Marine Institute, Galway, were analysed. Samples were extracted from 3 sites (inner, middle and outer) from Killary Harbour, Ireland, in 2009 and 2010. Mussels from a DSP event were tested by Mouse Bioassay (MBA), ELISA (Abraxis), LC-MS and protein phosphatase PP2A assay (OkaTest).
ELISA kits showed matrix effects, but PP2A technique (OkaTest, ZEU-INMUNOTEC) was recommended as rapid assay.
More information on http://www.nppwater.com/data_access.php?id=85