Zeulab News

Time-out for the mouse bioassay

Jan 13, 2015 1:09:11 PM / by admin posted in diarrhetic shellfish poisoning, lc mass, lc ms, lc msms, lipophilic marine toxins, lipophilic toxins, Marine biotoxins, Marine toxins, mass spec, mouse assay, okadaic acid, okadaic acid pp2a, Okatest, PP2A, pp2a inhibitor, shellfich food poisoning, shellfish poisoning, shellfish toxin, the liquid chromatography mass spectrometry, toxic shellfish poisoning

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time-out-for-the-mouse-bioassay,-okatest,-zeulab,-Marine-toxins-analysis-in-bivalve-molluscs

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Mouse bioassay for determination of lipophilic marine toxins in shellfish comes to its end

Jul 3, 2014 2:09:08 PM / by admin posted in diarrhetic shellfish poisoning, lc mass, lc ms, lc msms, lipophilic marine toxins, lipophilic toxins, Marine biotoxins, Marine toxins, mass spec, mouse assay, okadaic acid, okadaic acid pp2a, Okatest, PP2A, pp2a inhibitor, shellfich food poisoning, shellfish poisoning, shellfish toxin, the liquid chromatography mass spectrometry, toxic shellfish poisoning

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The presence of marine toxins in shellfish products could lead to fatal food poisoning. Monitoring programs to control levels of toxins establish monthly or even weekly sampling in production areas. Opening or closure of harvest areas would depend upon  the results obtained. Post-harvest and end product testing is also carried out in some countries. Currently, paralytic (saxitoxins), ammesic  (domoic acid) and lipophilic toxins (okadaic acid and derivatives, yessotoxins, pectenotoxins and azaspirazids) are legislated.

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A SLV of a PP2A developed by ZEU-INMUNOTEC, just published on Toxins journal

May 3, 2012 9:33:45 AM / by admin posted in DSP toxins, Marine biotoxins, Marine toxins, OA toxins, okadaic acid, Okatest, phosphatase, PP2A, protein phosphatases, SLV

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Single Laboratory Validation of A Ready-to-Use Phosphatase Inhibition Assay for Detection of Okadaic Acid Toxins

Toxins. 2012, 4, 339-352; http://www.mdpi.com/2072-6651/4/5/339/pdf
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A comparison of assay techniques for Okadaic acid toxins determination in shellfish

Jan 23, 2012 9:40:16 AM / by admin posted in DSP toxins, Marine biotoxins, Marine toxins, okadaic acid, Okatest

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PP2A (protein phosphatase) technique appears satisfactory for the analysis of DSP toxins in shellfish and is recommended as a rapid assay method for screening and end product testing.
This is one of the conclusions raised from a study carried out within the water project http://www.nppwater.com/index.php
Mussel samples from the biotoxins monitoring program carried by the Irish Marine Institute, Galway, were analysed. Samples were extracted from 3 sites (inner, middle and outer) from Killary Harbour, Ireland, in 2009 and 2010. Mussels from a DSP event were tested by Mouse Bioassay (MBA), ELISA (Abraxis), LC-MS and protein phosphatase PP2A assay (OkaTest).
ELISA kits showed matrix effects, but PP2A technique (OkaTest, ZEU-INMUNOTEC) was recommended as rapid assay.
More information on http://www.nppwater.com/data_access.php?id=85

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