Zeulab News

Time-out for the mouse bioassay

Jan 13, 2015 1:09:11 PM / by admin posted in diarrhetic shellfish poisoning, lc mass, lc ms, lc msms, lipophilic marine toxins, lipophilic toxins, Marine biotoxins, Marine toxins, mass spec, mouse assay, okadaic acid, okadaic acid pp2a, Okatest, PP2A, pp2a inhibitor, shellfich food poisoning, shellfish poisoning, shellfish toxin, the liquid chromatography mass spectrometry, toxic shellfish poisoning

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Moving away from Mouse Bioassay (MBA). Time to implement the new legistation for lipophilic toxins will end on 31 December 2014

Oct 7, 2014 9:32:59 AM / by admin posted in analysis by mass, bioassay systems, diarrheic shellfish poisoning, diarrhetic shellfish poisoning, algal toxins, harmful algae, inhibitor assay, lc mass, lc ms, lc msms, liquid chromatography mass spectrometry, Marine biotoxins, mass spec, mass spectroscopy, mba, mouse assay, mouse bioassay, okadaic acid, okadaic acid pp2a, Okatest, phosphatase assay, PP2A, pp2a inhibitor, red tie, shelfish poisoning symptoms, shellfish food poisoning, shellfish poisoning, shellfish toxin, spectrometry chromatography, toxic algae, toxins in shellfish

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3 months left to stop mouse bioassay for determination of lipophilic marine toxinsThe liquid chromatography-tandem mass spectrometry (LC-MS/MS) is the reference method for lipophilic toxins since July 2011. According to the European Commission EU 15/2011 this method has to be used as matter of routine, both for the purposes of official controls, at any stage of the food chain, and own-checks by food business operators. This legislation applies to European countries and any other exporting to Europe.

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Mouse bioassay for determination of lipophilic marine toxins in shellfish comes to its end

Jul 3, 2014 2:09:08 PM / by admin posted in diarrhetic shellfish poisoning, lc mass, lc ms, lc msms, lipophilic marine toxins, lipophilic toxins, Marine biotoxins, Marine toxins, mass spec, mouse assay, okadaic acid, okadaic acid pp2a, Okatest, PP2A, pp2a inhibitor, shellfich food poisoning, shellfish poisoning, shellfish toxin, the liquid chromatography mass spectrometry, toxic shellfish poisoning

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The presence of marine toxins in shellfish products could lead to fatal food poisoning. Monitoring programs to control levels of toxins establish monthly or even weekly sampling in production areas. Opening or closure of harvest areas would depend upon  the results obtained. Post-harvest and end product testing is also carried out in some countries. Currently, paralytic (saxitoxins), ammesic  (domoic acid) and lipophilic toxins (okadaic acid and derivatives, yessotoxins, pectenotoxins and azaspirazids) are legislated.

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The PP2A test- OKATEST- suggested as the screening test of choice for Washington State monitoring program.

Oct 30, 2013 10:29:45 AM / by admin posted in Marine biotoxins, Okatest

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A recent study at the Northwest Fisheries Science Center has evaluated the application of screening assays for determination of DST
(diarrhetic shellish toxins) in shellfish. A lateral flow test, ELISA and PP2A tests were evaluated and compared with the standard regulatory LC-MS/MS (Eberhart et al, Marine Drugs, 2013).

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Demonstration of OkaTest for Okadaic Acid-Toxins at Quasimeme Workshop

Jun 21, 2012 2:37:44 PM / by admin posted in lipophilic toxin testing, Marine biotoxins, marine toxins testing, okadaic acid testing, Okatest, phosphatase inhibition assay, protein phosphatases

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A SLV of a PP2A developed by ZEU-INMUNOTEC, just published on Toxins journal

May 3, 2012 9:33:45 AM / by admin posted in DSP toxins, Marine biotoxins, Marine toxins, OA toxins, okadaic acid, Okatest, phosphatase, PP2A, protein phosphatases, SLV

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Single Laboratory Validation of A Ready-to-Use Phosphatase Inhibition Assay for Detection of Okadaic Acid Toxins

Toxins. 2012, 4, 339-352; http://www.mdpi.com/2072-6651/4/5/339/pdf
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The European Reference Lab for Marine Biotoxins (EURLMB) informs that OkaTest fulfills the legislation requirements for OA-toxins testing

Mar 6, 2012 11:40:23 AM / by admin posted in Marine biotoxins, Okatest

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OkaTest, based on a phosphatase inbhibition assay and manufactured by ZEU-INMUNOTEC, fulfils  the Regulations (2074/2005 and 15/2011) requirements and can be used for detection of Okadaic Acid group toxins (OA, DTX1,DTX2, DTX3 including their esters).

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A comparison of assay techniques for Okadaic acid toxins determination in shellfish

Jan 23, 2012 9:40:16 AM / by admin posted in DSP toxins, Marine biotoxins, Marine toxins, okadaic acid, Okatest

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PP2A (protein phosphatase) technique appears satisfactory for the analysis of DSP toxins in shellfish and is recommended as a rapid assay method for screening and end product testing.
This is one of the conclusions raised from a study carried out within the water project http://www.nppwater.com/index.php
Mussel samples from the biotoxins monitoring program carried by the Irish Marine Institute, Galway, were analysed. Samples were extracted from 3 sites (inner, middle and outer) from Killary Harbour, Ireland, in 2009 and 2010. Mussels from a DSP event were tested by Mouse Bioassay (MBA), ELISA (Abraxis), LC-MS and protein phosphatase PP2A assay (OkaTest).
ELISA kits showed matrix effects, but PP2A technique (OkaTest, ZEU-INMUNOTEC) was recommended as rapid assay.
More information on http://www.nppwater.com/data_access.php?id=85

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